Fig 1: EMT markers in ARPE-19 cells. (A) ACTA2 and (B) Fn1 mRNA and protein levels. ACTA2 and Fn1 relative mRNA levels are reported as 2^-??Ct ± SD and quantized by using GAPDH as control. ACTA2 and Fn1 protein levels are reported as ng/mL ± SD of 9 observations per group (three independent experiments, each done in triplicate). ** p < 0.01 vs. NG; ° p < 0.05 and °° p < 0.01 vs. HG; NG, normal glucose (5 mM) + vehicle; M, mannitol (30 mM); HG, high glucose (35 mM) + vehicle; OTX 2.5, OTX 2.5 µM; OTX 5, OTX 5 µM; OTX 10, OTX 10 µM.
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